Insertion junction: CLIP2.063580_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.063580
Chromosome:chromosome 7
Location:1150804
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g320900 (1 of 1) PTHR20883//PTHR20883:SF5 - PHYTANOYL-COA DIOXYGENASE DOMAIN CONTAINING 1 // SUBFAMILY NOT NAMED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGAAGTGAGGATGCTCCATGACTGGTTGAGGAAATTGGGCCGGTCAGG
Internal bar code:ATCGACACCCACTAATGGGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:6086
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:128
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:128

Suggested primers for confirmation by PCR

Suggested primer 1:ATTTCCATGGCCACTCCCTG
Suggested primer 2:AGAGTGAAGTGGTGCTGACG