Insertion junction: CLIP2.063583_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.063583
Chromosome:chromosome 2
Location:8161570
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre02.g144006 (1 of 17) PF13833 - EF-hand domain pair (EF-hand_8) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGACTATTGTCGCGTGTCTCCGTTGTCAAGCATCGGCCACGCTCTCCTC
Internal bar code:CTAAACACCGTGCCTAGACGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:360
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGGATTTGCACCACCCCATA
Suggested primer 2:GCGTGAGAGCATGAAACGAC