| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063598 |
| Chromosome: | chromosome 12 |
| Location: | 2621272 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506000 | IC69,ODA6,ODA-IC2,DIC2,IC70,IC2 | (1 of 1) K11143 - dynein intermediate chain 2, axonemal (DNAI2); Flagellar Outer Arm Dynein Intermediate Chain 2 | 5'UTR |
| Cre12.g506050 | (1 of 1) K14408 - cleavage stimulation factor subunit 3 (CSTF3, RNA14) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCACATGTAATTATGCCTAACGATCTAGCTCTCAAGATTTAGAAAATG |
| Internal bar code: | CCATCGTCGGACGGGTACTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1756 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGGCTGCTTAGCTCCTA |
| Suggested primer 2: | TGCTTTCAGCTCCAAAACGC |