| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.063601 |
| Chromosome: | chromosome 3 |
| Location: | 1025631 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g148100 | (1 of 1) PTHR10281:SF4 - NEUFERRICIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGCGCTCTCTCATACGCTCCTCCAGGAAATCACGTCCCCACTTGCTG |
| Internal bar code: | CGGGCTCAAGAGTAGTTAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 567 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAGCTCACCGTTCATCC |
| Suggested primer 2: | AATTTTCACGGTTGCGTGGG |