| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.063603 |
| Chromosome: | chromosome 12 |
| Location: | 7554736 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556200 | (1 of 1) PF15011 - Casein Kinase 2 substrate (CK2S) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAGTCGCCCGTTTCCAAAACGCACGCTTCTTGTCTTCAAGCTCCGTG |
| Internal bar code: | TTTGCCTCGCACGTCCTATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3220 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGTCGAAGGAGGAGGAC |
| Suggested primer 2: | TCTCACGCCACAACATCGAA |