| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.063607 |
| Chromosome: | chromosome 17 |
| Location: | 3362126 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723000 | 5'UTR | ||
| Cre17.g723050 | (1 of 8) PTHR23023//PTHR23023:SF130 - DIMETHYLANILINE MONOOXYGENASE // INDOLE-3-PYRUVATE MONOOXYGENASE YUCCA1-RELATED | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTCGGCGGCTGTGGGCGGGTCACCAGATTTCGTTCATGACTGTGTCC |
| Internal bar code: | CGGGGGGGGCATGTTCCAATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2700 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCAGCCCATTTGCATTT |
| Suggested primer 2: | CCGTTAGGGCCCTGTTCATT |