| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.063624 |
| Chromosome: | chromosome 12 |
| Location: | 234277 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484950 | outside_mRNA | ||
| Cre12.g485000 | FAP182 | (1 of 2) PTHR20899:SF1 - UPF0691 PROTEIN C9ORF116; Flagellar Associated Protein 182 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCTACAACATAGCGCTGCGCGGTATTGTACGTTGCTGCCACTACGTG |
| Internal bar code: | GCCAGTGGTATGTGCATCTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4475 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTACGGACAGGTCGTGTG |
| Suggested primer 2: | CTACAGCTCATCCTCGCAGG |