Insertion junction: CLIP2.063643_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.063643
Chromosome:chromosome 12
Location:3096636
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g801388 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGCCGACGCCCCCAGAATCCAAATGTGACGAACCCAATAACCCAATGA
Internal bar code:GGATGACTGGGGGACGTGTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1663
LEAP-Seq percent confirming:76.4706
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:68

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGAGCCCACTCCTTCTTT
Suggested primer 2:GCACTGCTTGTTCGAGTGTG