| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.063645 |
| Chromosome: | chromosome 9 |
| Location: | 3263053 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394954 | (1 of 1) K03975 - membrane-associated protein (dedA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGCAGCAAAGAGCGGGGCGGATTTACCCCGGTGCTCGCTGCTGTAAC |
| Internal bar code: | TTAATTTAGTGGAATTGAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1296 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCACAGTGATGAACCCTG |
| Suggested primer 2: | GCAAGCCCAACTTTGCATGA |