Insertion junction: CLIP2.063672_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.063672
Chromosome:chromosome 9
Location:3066372
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g393913 (1 of 1) 3.5.4.16 - GTP cyclohydrolase I 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAACAGGCAACTGCTTCAGAAAGCGACACACTCGATCACCTCTGCCCAT
Internal bar code:CGGCCTGGCGCTCCCTTATGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:4126
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTGAGCTACTGATCGCAT
Suggested primer 2:TTGCTAGGCAGTGACAGTGG