| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063672 |
| Chromosome: | chromosome 9 |
| Location: | 3066373 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393913 | (1 of 1) 3.5.4.16 - GTP cyclohydrolase I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTTTTCGGGAGACTTGGGCAGGGCCCGGTGCATGGGATTGTTTTTTCG |
| Internal bar code: | AGTAGATATTGTCACGTCTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2091 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTAGGCAGTGACAGTGG |
| Suggested primer 2: | CGGTGAGCTACTGATCGCAT |