Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.063679 |
Chromosome: | chromosome 6 |
Location: | 1237552 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g258600 | (1 of 2) K01061 - carboxymethylenebutenolidase (E3.1.1.45) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTGGAGGGGGCGGAGGAGCAGCAAGGCGCTCCTGAAGTGGCGCCAGCA |
Internal bar code: | CGATTCGCACTTTCGGGTGAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3828 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 23 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGTCGACAAGCCCCAAGAA |
Suggested primer 2: | GCGTGTGTGAGTGTGAGAGA |