Insertion junction: CLIP2.063690_4

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.063690
Chromosome:chromosome 16
Location:1158678
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCAAGGTCGCAAGGATTCTATCGTAACGCCAGCACACGAGTAGCACG
Internal bar code:GTCAATCTTAAGGTTGCACTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1912
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGCATGTAAGCATCCAG
Suggested primer 2:TTGTAGTGGGCGTGTGAGTC