Insertion junction: CLIP2.063715_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.063715
Chromosome:chromosome 7
Location:2946691
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g332800 (1 of 1) K14544 - U3 small nucleolar RNA-associated protein 22 (UTP22, NOL6) 3'UTR
Cre07.g332851 SELM2,SELENOM,SELM (1 of 1) PTHR13077:SF7 - SELENOPROTEIN M; Selenoprotein M 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGTAGTGACAGTCTACTGGAAAGGGGCGTTAGAAACCGGACATCATTG
Internal bar code:CGTGAATGATTGCGTAACTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3652
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTGACTTCAACGTCCTCA
Suggested primer 2:CGTAACCACGTCCTCCAACA