| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.063733 |
| Chromosome: | chromosome 10 |
| Location: | 6370200 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464133 | 3'UTR | ||
| Cre10.g464200 | (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCAATAAGTAAACGGGCTGCTTAGTAATCACGATAGCAACGCAGAAGC |
| Internal bar code: | TGCCTGGTATCGGCCAGCTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 266 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCGAGAAGGACAGAGAGA |
| Suggested primer 2: | TGTGCCGTGATGCTCTTGAT |