| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.063801 |
| Chromosome: | chromosome 5 |
| Location: | 3600426 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800580 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACACACGGCACCCACGTTCAACAGCACTAACGCCTGCTGAGCATCT |
| Internal bar code: | GCTTCGTACTTTAGGGTAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4045 |
| LEAP-Seq percent confirming: | 98.8506 |
| LEAP-Seq n confirming: | 86 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCCCCATTCACCATCAT |
| Suggested primer 2: | AGGGTAGCACGCAAGAGTTC |