Insertion junction: CLIP2.063811_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.063811
Chromosome:chromosome 2
Location:4503068
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g106650 CDPK7 (1 of 6) PTHR24349//PTHR24349:SF23 - SERINE/THREONINE-PROTEIN KINASE // MAP KINASE-ACTIVATED PROTEIN KINASE 5; Calcium/calmodulin-dependent protein kinase 3'UTR
Cre02.g106700 OPR4,RAP1 OctotricoPeptide Repeat protein 4; (1 of 19) PTHR21228//PTHR21228:SF20 - FAST LEU-RICH DOMAIN-CONTAINING // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGCCACGGCCTAGCGACATGGTCGTGGGCAACGGGTCCAGGGGACAT
Internal bar code:GGATCCTGGATAAGCTCTCTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2411
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTGCGTGTTTCGTCCATA
Suggested primer 2:CGGAGATGAGGCAGATGCTT