| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.063819 |
| Chromosome: | chromosome 16 |
| Location: | 7396975 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680454 | (1 of 8) PF13417 - Glutathione S-transferase, N-terminal domain (GST_N_3) | 3'UTR | |
| Cre16.g680566 | (1 of 2) K17506 - protein phosphatase 1L [EC:3.1.3.16] (PPM1L, PP2CE) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCTCGATAGCGATAGCGCGTGATTGGGAGTGTGTGTGCTGCAGCGTG |
| Internal bar code: | TGGCAACAGTGTCATGGGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3289 |
| LEAP-Seq percent confirming: | 98.4127 |
| LEAP-Seq n confirming: | 124 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 126 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGAATCTCCACGCGTCA |
| Suggested primer 2: | CACGGGAAGTTCGGAGTGAA |