| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063821 |
| Chromosome: | chromosome 10 |
| Location: | 2782167 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438450 | LTH1 | LAT2 homolog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTAAGCACTCCTGACTCGCTCATGCATGGAACTGGTCATTGTCGAAGG |
| Internal bar code: | CGGTTAAGTGCATTGTTACAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5438 |
| LEAP-Seq percent confirming: | 97.2603 |
| LEAP-Seq n confirming: | 142 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 146 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGACATGGAGTGGTGCC |
| Suggested primer 2: | CGGCTATGTCAATGCGTTGG |