Insertion junction: CLIP2.063855_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.063855
Chromosome:chromosome 12
Location:1235022
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g490050 TPT18,GMT1,TPT19 (1 of 3) K15356 - GDP-mannose transporter (VRG4, GONST1); Putative GDP-mannose transporter 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTACATTTGACCGCAATCTTGACAGGTCGACGCTGCGACGTTTTCGACTG
Internal bar code:TAGGATGGTCGGGACTAAACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2068
LEAP-Seq percent confirming:97.0588
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CCACTTGACAAAACCAGCGG
Suggested primer 2:GAATCCCAGTCCAGCAGCTT