Insertion junction: CLIP2.063859_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.063859
Chromosome:chromosome 1
Location:698067
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g003850 CYP1,CYP747A1 Cytochrome P450, CYP197 superfamily; (1 of 19) 1.14.14.1 - Unspecific monooxygenase / Xenobiotic monooxygenase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCAAGTGCCAGACCCCATGCCCCAAAATCCCGGCCGGGACCCCCCCTG
Internal bar code:TGTAGGTTAAGTTCGACATATG

Confirmation - LEAP-Seq

LEAP-Seq distance:96
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TAGATGTTGCTGCTGTGGGG
Suggested primer 2:CAGGGTATACGAGACGTGGC