Insertion junction: CLIP2.063869_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.063869
Chromosome:chromosome 2
Location:2105160
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g089100 COPD1 (1 of 1) PTHR10121 - COATOMER SUBUNIT DELTA; Delta subunit of COP-I complex outside_mRNA
Cre02.g089150 (1 of 1) K11314 - transcriptional adapter 2-alpha (TADA2A, ADA2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCAGCTCACACACCGCTATGTTCATCCTCTCTCACTCCCAACCTGGCA
Internal bar code:TGAACGGCGAGAGTGCTATCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3516
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTGTACCCCCATCCTGTA
Suggested primer 2:CAACACACACATGCACCAGG