| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.063873 |
| Chromosome: | chromosome 9 |
| Location: | 571130 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g748447 | NFU1 | (1 of 3) PF01106 - NifU-like domain (NifU); Iron-sulfur cluster assembly protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGTGGCTGGTGTTCTTCAATTACGTCTACGTATATCTGCGACTGTAT |
| Internal bar code: | CTAATTCAGGCGGAAGGCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 815 |
| LEAP-Seq percent confirming: | 76.4706 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCATTCACCCCATGAGA |
| Suggested primer 2: | CACCACCACTCACATCTCCC |