| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.063890 |
| Chromosome: | chromosome 6 |
| Location: | 2184170 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266350 | (1 of 1) PF00400//PF01753//PF12937 - WD domain, G-beta repeat (WD40) // MYND finger (zf-MYND) // F-box-like (F-box-like) | 5'UTR | |
| Cre06.g266383 | HEL26 | (1 of 1) K18995 - ATP-dependent RNA helicase DHX29 [EC:3.6.4.13] (DHX29); DEAD/DEAH box helicase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGAGACGCCAAATCCTTATTAGATAACCCTGTGTTTGGCTCGCGGCGA |
| Internal bar code: | TACGGTCAGAGACGGTTCAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1491 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAGTAGAAGCAAGGCGGG |
| Suggested primer 2: | GTCCTTTTGGCCTGTGCATG |