| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063893 |
| Chromosome: | chromosome 7 |
| Location: | 1512044 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g324200 | BTA1 | (1 of 1) K13621 - betaine lipid synthase (BTA1); Diacylglyceryl-N,N%252CN-trimethylhomoserine synthesis protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCTAGCCACCCGCAGGCTATCGCCCCAGGCCTGCAGTATTGCATCGC |
| Internal bar code: | TGTGTCTTTGAGCTGGTTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2651 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCCGTGTAGCGTTACAA |
| Suggested primer 2: | TGTGCACACCAGACGAGTAC |