| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.063931 |
| Chromosome: | chromosome 10 |
| Location: | 3938094 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446750 | (1 of 2) PTHR13690//PTHR13690:SF71 - FAMILY NOT NAMED // PROTEIN C37C3.9 | outside_mRNA | |
| Cre10.g446800 | (1 of 3) IPR016087 - Chalcone isomerase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGCCCATATAGCACTTCCTCCCCTTCACAGAGCATATACAGTCTGTT |
| Internal bar code: | ACACCGTGCCGAGTCGTAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2308 |
| LEAP-Seq percent confirming: | 75.8621 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGCCGCTTTAGCAAATC |
| Suggested primer 2: | GCAAACTGTCGGTTTCCACC |