Insertion junction: CLIP2.063945_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.063945
Chromosome:chromosome 8
Location:1594079
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre08.g365632 PPP32 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCTCCACCACGCCAAACAGCCGCTTCTGCAGCCAATCCGCAGTGGCGA
Internal bar code:TGATCTACATCCTTGTTATGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:170
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGCCCTTGCTCAGAAGTC
Suggested primer 2:GAAAGTGGGAGATGGGGGTG