| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.063949 |
| Chromosome: | chromosome 3 |
| Location: | 485945 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145007 | CDS | ||
| lncRNA_TCONS_00148410 | CDS | ||
| lncRNA_TCONS_00148950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGAGGACAAGATCATCCTGCAGTTCCTGAGCCCAGCAGGCCCCACGG |
| Internal bar code: | TGGGGTGGGCGTTTATCTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 712 |
| LEAP-Seq percent confirming: | 94.2857 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATCGTAGGAGTGCCTGGG |
| Suggested primer 2: | CAGGTGGGTGCATTTTGTGG |