| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.063964 |
| Chromosome: | chromosome 6 |
| Location: | 5414796 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285600 | MID,RWP5 | (1 of 16) PF02042 - RWP-RK domain (RWP-RK); RWP-RK transcription factor | 3'UTR |
| Cre06.g285650 | ORC6 | (1 of 1) PF05460 - Origin recognition complex subunit 6 (ORC6) (ORC6); Origin recognition complex subunit 6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACTCAGTACAGCCCACCCTCGCCAGCGGACTGCCACTGCCCAGCCGAC |
| Internal bar code: | TCTTATCGCTGAGGTTTTATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2559 |
| LEAP-Seq percent confirming: | 79.4118 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTTTGGTTCGTCCGTGG |
| Suggested primer 2: | GTTATGAGGGTGTTGGGCGA |