Insertion junction: CLIP2.063976_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.063976
Chromosome:chromosome 4
Location:2896537
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g224500 (1 of 1) 3.6.3.17//3.6.3.44 - Monosaccharide-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGACATGCCGCGGAAACCGCCAGGTTGTCACGGAGGTAGTAACGATG
Internal bar code:CGCATTCGCGCTGGTTCGTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1156
LEAP-Seq percent confirming:44.8276
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCCTTCGACCAGTTGCTG
Suggested primer 2:TGCTCCCTCTGAACAGCATG