| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.063986 |
| Chromosome: | chromosome 7 |
| Location: | 5630969 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352350 | FHL7,FTSHI6,FHL3,FTSHi6 | (1 of 6) K03798 - cell division protease FtsH [EC:3.4.24.-] (ftsH, hflB); Chloroplast-import FtsH-like ATPase | 5'UTR |
| Cre07.g352400 | IPP5 | Inositol polyphosphate related phosphatase; (1 of 2) 3.1.3.56 - Inositol-polyphosphate 5-phosphatase / Type I inositol-polyphosphate phosphatase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAGGGCTCCAAGCGCCTATGGACCAAAGTGGTCTTTGCATGAAGGAGG |
| Internal bar code: | CGAATTCGATTTACACAAACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 88.6364 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGCTGCGCTCCTTACTAC |
| Suggested primer 2: | AAGCATTGTTTCCCGCGTTC |