| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.063994 |
| Chromosome: | chromosome 9 |
| Location: | 1016227 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402300 | ASA8 | Mitochondrial F1F0 ATP synthase associated protein 8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCGTCAGCAGGTCCCTCTTGTAACCAATGCAATCTCTTGCGCCCTTGC |
| Internal bar code: | AATAATCAGGGAATGATAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4417 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 147 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 147 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAGAAGCTGTTCCCTCG |
| Suggested primer 2: | GGTGAGAAGCAAAGTCCCGA |