| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.064001 |
| Chromosome: | chromosome 2 |
| Location: | 7807106 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146450 | CYG60 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCCCGAAAAGCAAGCCATGGCTTAGCGCAGTATGACTTCTGTACGTC |
| Internal bar code: | CTGATAGCTTCATTTACTCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3431 |
| LEAP-Seq percent confirming: | 79.6296 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGCCAAGCAACGCAAAG |
| Suggested primer 2: | GACTCGTGCTGGTATACCGG |