Insertion junction: CLIP2.064024_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.064024
Chromosome:chromosome 16
Location:7363688
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre16.g679669 CMS1 4-diphosphocytidyl-2C-methyl-D-erythritol synthase, chloroplast precursor; (1 of 1) 2.7.7.60 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase / MEP cytidylyltransferase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGTTTAAAGTAGTAACTATTACACATACGAAAGTAGACAGGGGAACTG
Internal bar code:GGATATACAAACTAGGTAAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:647
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCAAACAAAACTACGGCA
Suggested primer 2:GCACACGATGACAATCTCGC