Insertion junction: CLIP2.064025_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.064025
Chromosome:chromosome 11
Location:2188531
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467727 (1 of 1) IPR000104//IPR002711//IPR003615//IPR004919 - Antifreeze protein, type I // HNH endonuclease // HNH nuclease // Domain of unknown function DUF262 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGGTCGGTTCCGCGAAGGTTGACACGCGGCAAACACCCAGCGTGCACG
Internal bar code:TAGGTCTATGTTCTTGTTGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2090
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CCAACCCGGCAGTCTTATGT
Suggested primer 2:TTGGATTGCAAGGAAACGCG