| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064025 |
| Chromosome: | chromosome 11 |
| Location: | 2188540 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467727 | (1 of 1) IPR000104//IPR002711//IPR003615//IPR004919 - Antifreeze protein, type I // HNH endonuclease // HNH nuclease // Domain of unknown function DUF262 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCCGCCCCAATATGTTTCCCGAAATATTTAGTGCATGATTCAACCCT |
| Internal bar code: | TAGGTCTATGTTCTTGTTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6389 |
| LEAP-Seq percent confirming: | 98.3607 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGATTGCAAGGAAACGCG |
| Suggested primer 2: | CCAACCCGGCAGTCTTATGT |