Insertion junction: CLIP2.064029_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.064029
Chromosome:chromosome 6
Location:4975652
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre06.g281400 NAH1 Cation antiporter; (1 of 11) PF00999 - Sodium/hydrogen exchanger family (Na_H_Exchanger) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCGGCGGCGGCGACGGCGGCGAGGAGCGGTGTGGCGAAGGCAGCTGGA
Internal bar code:TTGAGCTATCACATCATCCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1914
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCCCAGTGCTGTATCTG
Suggested primer 2:GTTCAAAGGTGCAACGACCC