Insertion junction: CLIP2.064031_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.064031
Chromosome:chromosome 3
Location:6647361
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g195100 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTACTGCCGTGTAGCGCTGCAAACCGCCACGGCCCAAACGACAACCCAA
Internal bar code:CAGTTGGGTAGGATGATATAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1122
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTCAGATGGCGACCATA
Suggested primer 2:CGTCCGCAGTCCTGTATACC