| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.064034 |
| Chromosome: | chromosome 1 |
| Location: | 5258922 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036900 | (1 of 2) K04427 - mitogen-activated protein kinase kinase kinase 7 (MAP3K7, TAK1) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCGGACAAAACCCACCCCAAACCCGGGCCAACAAAGTCTCGCCCCAG |
| Internal bar code: | TGGTAGCGTAAACTTATTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2135 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACGTTGCATGTTGTCGTT |
| Suggested primer 2: | ATTCCTTACCCTCCCTCCCC |