Insertion junction: CLIP2.064047_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.064047
Chromosome:plastome
Location:62662
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802290 ChreCp026,2716975,psbM photosystem II protein M; (1 of 1) K02714 - photosystem II PsbM protein (psbM) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTACACTGCTCTTAATATGTCTGTTAGAGCATACACTTCACAGTATTT
Internal bar code:TGTACTGTGCGTCATTTCTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:607
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGTTGGACACAGGTTGCT
Suggested primer 2:ATGTGATGCCTCGCCTATCG