| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.064053 |
| Chromosome: | chromosome 6 |
| Location: | 7972785 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304500 | ZYS3-2,EZY9,ZYS4 | (1 of 2) PF00397//PF12796 - WW domain (WW) // Ankyrin repeats (3 copies) (Ank_2); Zygote-specific protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTTGTAGCATGACAGCTGGTCAAAAGGCATCTCCAGGTCCGCTTCTA |
| Internal bar code: | CGTGTAAGGACCCGGACGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2718 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCCCTCACCTTCTTTCC |
| Suggested primer 2: | GGGCAGTTGCAGTACCTGAT |