Insertion junction: CLIP2.064085_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.064085
Chromosome:chromosome 3
Location:7247355
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g207700 FPPS1,FPPS,PPS1,FPS1 (1 of 1) 2.5.1.1//2.5.1.10 - Dimethylallyltranstransferase / Prenyltransferase // (2E,6E)-farnesyl diphosphate synthase / Geranyltranstransferase; Farnesyl pyrophosphate synthase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACTCGTGTCTGCGTGAGAAGGGTTTCGGGGGGAGCGACCACGGATTC
Internal bar code:TAGTCAAGCGAAACAAGAACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3686
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:97
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:97

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGGTAACAGGTCACGAAA
Suggested primer 2:CTCATGGATAGCTGCAGGGG