Insertion junction: CLIP2.064085_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.064085
Chromosome:chromosome 3
Location:7247357
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g207700 FPPS1,FPPS,PPS1,FPS1 (1 of 1) 2.5.1.1//2.5.1.10 - Dimethylallyltranstransferase / Prenyltransferase // (2E,6E)-farnesyl diphosphate synthase / Geranyltranstransferase; Farnesyl pyrophosphate synthase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGCCGCAGGTGCCATCCGCGATCCATAACTTATTACGTGCGCAGGAAC
Internal bar code:GACCACTTCGGAGTGTTAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2899
LEAP-Seq percent confirming:89.899
LEAP-Seq n confirming:89
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:99

Suggested primers for confirmation by PCR

Suggested primer 1:CTCATGGATAGCTGCAGGGG
Suggested primer 2:CGGGGTAACAGGTCACGAAA