| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064101 |
| Chromosome: | chromosome 6 |
| Location: | 1858298 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263850 | PPT2,TPT2,TPT20 | Triose phosphate/phosphate translocator; (1 of 2) PTHR11132:SF70 - PHOSPHOENOLPYRUVATE/PHOSPHATE TRANSLOCATOR 1, CHLOROPLASTIC | gene_edge/outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCGTCGCACCCAATCCCTGACACGGGCACCATAACTCCGTAAACGCC |
| Internal bar code: | ATGGGGGGGTTACCAGGGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2066 |
| LEAP-Seq percent confirming: | 92.9412 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCGGATGGGTGCAATTGT |
| Suggested primer 2: | CCCTTCCAGTAGGCAGTGTG |