| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.064104 |
| Chromosome: | chromosome 12 |
| Location: | 7160321 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559450 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTGAGGGGTGAGGGGTGAAGGTTGCTAAAGGGCGCACCTCACCTCCC |
| Internal bar code: | CTTTGTTATACCAAAAATTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 397 |
| LEAP-Seq percent confirming: | 69.2308 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAGGAGAAGGGATGCAG |
| Suggested primer 2: | ACACAGAAATCCGCGACCAT |