| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.064120 |
| Chromosome: | chromosome 14 |
| Location: | 1674352 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618820 | CSB57 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon); {"Probable transposon-derived protein of Chlamydomonas-Specific family B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAATTGCTGGTGGGTGGGTTGAGTGGGATTGCAAGGTGGGTGGGTTGAG |
| Internal bar code: | AGATCCAGCGTAGTTATACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1298 |
| LEAP-Seq percent confirming: | 40.9091 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTATCAACCCAGCCACCT |
| Suggested primer 2: | GCAGCTGGATGAGGAGTTGT |