| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064129 |
| Chromosome: | chromosome 12 |
| Location: | 9693587 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556653 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTAAGCCTTCGCACCCCGGTGGGGTTTCGACAACTTCCGACAGGGCAC |
| Internal bar code: | ATGCATTGAGGTGGGGCGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2939 |
| LEAP-Seq percent confirming: | 73.3333 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGTAGCTTGCATCGTTC |
| Suggested primer 2: | TACAGTACGGCAGGGATGGA |