| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.064132 |
| Chromosome: | chromosome 12 |
| Location: | 4785647 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523300 | RPN1A,OST1A,GTR22 | Oligosaccharyltransferase subunit 1A, Ribophorin-1A; (1 of 1) K12666 - oligosaccharyltransferase complex subunit alpha (ribophorin I) (OST1, RPN1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAATGGGTGCACAGCGTCTGCCACGGTTCCGTCTGCCCTGCAAACAC |
| Internal bar code: | TCAAGTAAGAGCCTCGAGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1476 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCTATCGACACCGTGTT |
| Suggested primer 2: | GCTCACCTTCCACGGATTCA |