| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064168 |
| Chromosome: | chromosome 5 |
| Location: | 873211 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246250 | (1 of 12) IPR000104//IPR002893 - Antifreeze protein, type I // Zinc finger, MYND-type | 3'UTR | |
| Cre05.g246300 | (1 of 1) PTHR13554//PTHR13554:SF10 - 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5-RELATED // 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGTACGGACGGCCAAAACGTCCAGAAAAGGCTTGAAGGCTTTCGCAAG |
| Internal bar code: | TCGCCGAGTAAGGTTAGCGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1205 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGCGGTACAGTTTTT |
| Suggested primer 2: | GACGTGCAATTGCTAGCCTG |