| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.064173 |
| Chromosome: | chromosome 10 |
| Location: | 3053164 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441000 | (1 of 1) K17498 - transcription factor SPN1 (SPN1, IWS1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCCGTCTTGTGGGTTGTGTGTGCGCGTGTGAGTGCCTGTTTGCGGCG |
| Internal bar code: | TTACTATAGTAGCGCTATCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2902 |
| LEAP-Seq percent confirming: | 47.3684 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGAGGTGTTGTTTCCGAG |
| Suggested primer 2: | CTCGTGCTCGACTATCCACC |